Dr. Seema Dwivedi
Gautam Buddha University
Download PDFArsenic is the 20th most abundant element found universally on earth and it is consumed by the human through various means including drinking water, and food, etc. Arsenic also has a permissible limit within which it is safe but above that it is toxic and may harm human health. So it is very necessary to monitor the arsenic concentration in different stuffs of human use. Although there are many traditional techniques available that are being used from a long time to detect and estimate the arsenic level in drinking water and soil but they are costly, less efficient and also have toxic effect to environment since different chemicals are used in them. Hence developing efficient and eco-friendly detection and estimation tools is of great concern of today’s situation where arsenic toxicity is increasing day by day. Use of microbes as detection and estimation tool is showing great potential. In this review paper we have tried to discuss different methods of arsenic detection using engineered microorganism. Microbes can be engineered by inserting a number of different bioreporter genes including luxCBDAE, luxAB, and lacZ genes which makes microorganisms fluorescent that gives signal when they exposed to arsenic and that fluorescence signal is amplified and detected by a device called- bioluminescent bioreporter integrated circuits (BBICs). Such bio-based techniques have advantages over conventional methods in terms of more sensitive, more specific and less worthy.
Keywords: Arsenic, bioreporter, bioluminescence, luxCBDA, luxAB, lacZ
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