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PREPARATION AND EXTRACTION OF ANTIBIOTIC FROM OVULE OF CYCAS REVOLUTA

Dr. Seema Dwivedi

Gautam Buddha University

49-54

Vol: 6, Issue: 2, 2016

Receiving Date: 2016-02-20 Acceptance Date:

2016-03-18

Publication Date:

2016-04-20

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Abstract

Cycas revoluta basically belongs from member of cycad family. Cycas carcinalis was identified having antibacterial property against human pathogenic bacteria like . E.coli, Pseudomonas, Staphlyococcus aureus, Bacillus cerius. According to some source of information every part of the gymnosperm plant have antibacterial properties. This is a native plant of Southern Japan including Ryukyu island. The Cycas revoluta has three layers the exterior layer is called Sarcotesta. The middle layer is called Sclerotesta and the internal layer is called Endotesta. The various parts of the the plant is extracted with distil water, petroleum ether, methanol, ethanol, chloroform. All the extraction of various layers of the plant shows the antibacterial property aginst various human pathogenic bacteria (E.coli, Pseudomonas, Staphlyococcus aureus, Bacillus cerius). The zone of inhibition obtained in the antibacterial assay ranges from 15 to 28. A comparative analysis of inhibition activity of the extracts with antibiotics like Vancomycin and Penicillin against human pathogenic bacteria was made for testing of the higher antibacterial property.

Keywords: Cycas revoluta Ovule, Anti-bacterial Activity, Human Pathogenic Bacteria

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