Details

ANTIBACTERIAL ACTIVITY OF ZEA MAYS L AND URTICA DIOICA L EXTRACT ON THE ISOLATED BACTERIA FROM CHILDREN UTI IN ERBIL CITY

Sawsan Mohammed Abdullah Surjee

Biology Department, College of Education/Scientific Departments, University of Salahaddin, Erbil, Iraq.

Luma Abdal Hady Zwain

Biology Department, College of Education for Pure Science Ibn-Al-Haitham, University of Baghdad, Iraq

204-213

Vol: 5, Issue: 3, 2015

Receiving Date: 2015-07-02 Acceptance Date:

2015-08-03

Publication Date:

2015-09-05

Download PDF

Abstract

The antibacterial effect of ethanolic and aqueous extracts of Zea mays L and urtica dioica L, was investigated on bacteria were obtaind from human Urinary Tract Infection (UTI) from Hawlery Ferkary Hospital in Erbil City- Iraq, by using the well diffusion technique .A 120 isolates bacteria isolated from 180 UTA patient, isolates were identified as E.coli, Staph. spp, Staph. albus, Staph. capitis, Staph epidermis, Staph. aureus, Pseudo. spp., Pesudo. lutela, Pseudo. aeruginosa, Proteus. spp., P. mirabilis, Morganella, Morganii, Klebsiella spp., K. Pneumonia, K. oxytoca, Micrococcus and Citrobacter frenudii following different morphological, physiological and biochemical test. Antibiotic sensitivity of 17 commerical antibiotic discs AK,AMC, AMP, AT, ATM, AZM, CAZ, CD, CEP, CFM , CIP, COT, CRO, GM, IPM, MEM and NA was screened by disc diffusion assay , It was observed that the extracts of both plant had an inhibitory effect on the bacteria under study, except Pesudo. lutela to extracts of Zea mays L. and Pesudo. lutela, K. Pneumonia and K. oxytoca to extracts of Urtica dioica L. The inhibitory concentration of of Zea mays L. extracts was 75% and 100% and Urtica dioica L. extracts was 50%, 75% and 100%. The antibacterial activity of extracts of U. dioica showed the best action as inhibitor against test bacteria than the extracts of zea mays. The result showed there is antibacterial activity of alcohol extract of U. dioica higher sensitivity against a number of bacteria compared with standard antibiotics

Keywords: Antibacterial ; patient ; Pneumonia ; isolates

References

  1. Adwan,G.;S; Abu-Shanab,B. and Adwan, k. In vitro Interaction of Certain Antimicrobial Agents in Combination with Plant Extracts Against Multidrug-resistant Pseudomonas aeruginosa Strains. Middle-East J. Scient. Res., 4 (3): 158-162, 2009
  2. Akrayi, H.F.S. and Abdulrahman, Z.F.A. 2013. Evaluation of the antibacterial efficacy and the phytochemical analysis of some plant extracts against human pathogenic bacteria. JPCS, Vol (7).
  3. Akrayi, H.F.S. and Tawfeeq, J.D. 2012. Antibacterial activity of Lepidium sativum and Allium porrum extracts and juices against some gram positive and gram negative extracts. Med. J. Islam. World Acad. Sci., 20:1, 10-16.
  4. Al-Kareemi, K.K. 2012. Inhibitory Effect of Parsley (Petroselinum crispum) Juice Against Some Urinary Pathogens in vitro the Iraq Postgraduate med. J., 11(3).
  5. Bensky, D. and Gamble A. 1986. Chinese Herbal Medicine, Materia medica, Eastland Press, Seattle: Washington.
  6. Al-Wasfi, R.M.A.H., Al-Kaabee, H.J.J., Al-Fatlawy, D.M.H.D. 2012. Studying the hypoglycemic and the antibacterial activity of variousplant extract of Urtica dioica. Mag. Alkufa Univ.Biol., 4 (2 ) : 2012
  7. Cowan, M.M. 1999. Plant Products as Antimicrobial Agents. Americ. Soc. Microbio., 12(4):564-82.
  8. Elliger., C.A., Chan, G.B., Waiss, A.C.Jr., Lundin, R.E. and Haddon, W.F. 1980. Glycosylflavones from Zea mays that inhibit insect development. Phytochem., 19: 293-297.
  9. Fazilatun, N., Zhari, I. and Nornisah, M. 2001. Phytochemicals from corn silk (Zea mays). J. Trop. Med. Plants, 2: 189-192.
  10. Grieve, M. A. 1971. Modern Herbal. Dover Publication, New York.
  11. Guevara, P., Perez-Amador, M.C., Zuniga, B. and Snook, M. 2000. Flavones in corn silks & resistance to insect attacks. Phyton. Int. J. Exp. Bot., 69: 151–156.
  12. Guo, J., Liu, T., Han, L. and Liu, Y. 2009. The effects of corn silk onglycaemic metabolism Nutr Metab (Lond), 6: 47-52. Habtemariam, S. 1998. Extract of c
  13. necrosis factor-alpha- and bacterial lipopolysaccharide-induced cell adhesion and ICAM-1 expression. Planta Med., 64: 314-8.
  14. Johnson, M., Wesely, E.G., Selvan, N., Kavitha, M.S. 2010. In vivo and in vitro AntiBacterial Efficacy of Alternanthera sessilis (Linn.). Int. J. Pharma Res. and Develop.
  15. Kukrića, Z.Z., Topalić-Trivunovića, I.N., Kukavicab, B.M.; Matoša, S.B., Pavičića, S.S. Borojab, M.B. and. Savića, A.V. 2012. Characterization of antioxidant and antimicrobial activities of nettle leaves (Urtica dioica L.) Apteff, 43, 1-342.
  16. Maksimovic, Z.A., Malencic, D. and Kovacevic, N. 2005. Polyphenol contents and antioxidant activity of Maydis stigma extracts. Bioresour. Technol., 96: 873-7.
  17. Nessa, F., Ismail, Z. and Mohamed, N. 2012. Antimicrobial Activities of Extracts and Flavonoid Glycosides of Corn Silk (Zea mays L). Int. J. Biotechnol. Wellness Industries, 1:115-121
  18. Parekh, J., Chanda, S.V. 2008. Antibacterial Activity of Aqueous and Alcoholic Extracts of 34 Indian Medicinal Plants against Some Staphylococcus species. Turk. J. Biol., 32:63-71.
  19. Rahman, M.U., Gull, Sh., Odhano, E.A., Soomro, U.A. and Hafeez, I.A.F. 2010. Fectivity of Zataria multiflora boiss alcoholic extracts against bacteria. Int. J. Libyan Agr. Res. Cent., 1(3):147-152.
  20. Sharifian, M., Karimi, A., Tabatabaei, S.R., Anvaripour, N. 2006. Microbial sensitivity pattern in urinary tract infections in children: A single center experience of 1177 urine cultures. Jpn. J Infect Dis.,59: 380-82.
  21. Tambekar, D.H. and Dahikar, S.B. 2011. Antibacterial activity of some Indian Ayurvedic preparations against enteric bacterial pathogens. J. Adv. Pharm. Technol. Res., 2(1): 24–29.
  22. Tucakov, J. 1997. Lečenje biljem, Rad, Beograd:405 pp.
  23. Waiss, A.C., Chan, B.G. and Elliger, C.A. 1979.Maysin, a flavones glycoside from corn silks with antibiotic activity towards cor earworm. J. Econ. Entom., 72: 256-8.
Back

Disclaimer: All papers published in IJRST will be indexed on Google Search Engine as per their policy.

We are one of the best in the field of watches and we take care of the needs of our customers and produce replica watches of very good quality as per their demands.