A 0.5 ml aliquot was taken in test tube and mixed with 5 ml of reagent (c) solution allowed to stand for 10 minutes. Thereafter, 0.5 ml of reagent (d) was added with instant mixing. After 30 minutes absorbance was recorded at 570 nm through spectrophotometer (Model SL177) against reagent blank. Standard curve was prepared with a graded concentration of bovine-serum albumin. Similarly the residue left after soluble sugar extraction was suspended with 5 ml of distilled water and 6.5 ml of 52 per cent cold perchloric acid (40 C) for 12 hours. It was then centrifuged at 60007000 rpm for 20 minutes in a cooling centrifuge at 40C. The supernatant was collected in 100 ml volumetric flask. Extraction was repeated with 5.0 ml of distilled water and 6.5 ml of 52 per cent cold perchloric acid (40 C) and centrifuged as above. Both the supernatant were combined and the final volume was made up to 100 ml. 0.5 ml of aliquot was transferred in a test tube and 4.5 ml distilled water was added to it. 10 ml of anthrone reagent was added to each test tube, allowing the reagent to run down the side of the test tube. Mixed well and placed on a boiling water bath for exactly 8 minutes after which it was cooled to room temperature with ice water. The absorbance was measured at 630 nm against a reagent blank in spectrophotometer. Amount of starch was calculated using standard curve prepared from glucose.

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