Biomolecules are not limited to, proteins, carbohydrates, nucleic acids, and lipids. These molecules are
the basic building blocks of life and by controlling, creating, and manipulating their form and function
there are many new avenues and advantages available to society. Since every biomolecule is different,
there are a number of techniques used to manipulate each one respectively. Proteins are polymers that
are made up of amino acid chains linked with peptide bonds. They have four distinct levels of structure:
primary, secondary, tertiary, and quaternary. Carbohydrates are another important biomolecule. These
are polymers, called polysaccharides, which are made up of chains of simple sugars connected
via glycosidic bonds. These monosaccharides consist of a five to six carbon ring that contains carbon,
hydrogen, and oxygen - typically in a 1:2:1 ratio, respectively. Nucleic acids are macromolecules that
consist of DNA and RNA which are biopolymers consisting of chains of biomolecules. These two
molecules are the genetic code and template that make life possible. Manipulation of these molecules and
structures causes major changes in function and expression of other macromolecules. Nucleosides are
glycosylamines containing a nucleobase bound to either ribose or deoxyribose sugar via a betaglycosidic linkage.
Common fatty acids include lauric acid, stearic acid, and oleic acid. The study and engineering of lipids
typically focuses on the manipulation of lipid membranes and encapsulation. Cellular membranes and
other biological membranes typically consist of a phospholipid bilayer membrane, or a derivative thereof
Download PDF
References
- ^ Cornell Chronicle, May 14, 1987, page 3.Biologists invent gun for shootingcells with DNA
- ^ Sanford JC et al (1987) Delivery of substances into cells and tissues using aparticle bombardment process. Journal of Particulate Science and Technology 5:27-37.
- ^ Klein, TM et al (1987) High-velocity micro projectiles for delivering nucleicacids into living cells. Nature 327:70-73.
- ^ Lee LY, Gelvin SB (February 2008). 'T-DNA binary vectors and systems'. Plant Physiol. 146 (2):325-332. doi:10.1104/pp.107.113001. OCLC 1642351.PMC 2245830. PMID 18250230.
- ^ Park F (October 2007). 'Lentiviral vectors: are they the future of animal transgenesis?'. Physiol. Genomics 31 (2):159–173.doi:10.1152/physiolgenomics.00069.2007. OCLC 37367250. PMID 17684037.
- ^ Jackson, DA; Symons, RH; Berg, P (1 October 1972). 'Biochemical Method forInserting New Genetic Information into DNA of Simian Virus 40: Circular SV40 DNA Molecules Containing Lambda Phage Genes and the Galactose Operon of Escherichia coli'. PNAS 69 (10):2904–2909. Bibcode:1972PNAS 69.2904J. doi:10.1073/pnas.69.10.2904. PMC 389671. PMID 4342968.
- ^ M. K. Sateesh (25 August 2008). Bioethics and Biosafety. I. K. International Pvt Ltd. pp. 456–. ISBN 978-81-906757-0-3. Retrieved 27 March 2013.
- ^ Jefferson R. A. Kavanagh T. A. Bevan M. W. (1987). 'GUS fusions: beta- glucuronidase as a sensitive and versatile gene fusion marker in higher plants'. EMBO journal 6(13): 3901– 3907. ISSN 0261-4189. PMC 553867. PMID 3327686.
- ^ Nosowitz, Dan (15 September 2011) Suntory Creates Mythical Blue (Or, Um, Lavenderish) Rose Popular Science, Retrieved 30 August 2012
- ^ a b c Phys.Org website. April 4, 2005 Plant gene replacement results in the world's only blue rose
- ^ Kyodo (11 September 2011 Suntory to sell blue roses overseas The Japan Times, Retrieved 30 August 2012
- ^ Wired Report 2011
- ^ Gasdaska JR et al (2003) Advantages of Therapeutic Protein Production in the Aquatic Plant Lemna. Bio Processing Journal Mar/Apr 2003 pp 49–56 [1]
- ^ (10 December 2012) Engineering algae to make complex anti-cancer 'designer' drug PhysOrg, Retrieved 15 April 2013
- ^ Büttner-Mainik, A., et al (2011): Production of biologically active recombinant human factor H in Physcomitrella. Plant Biotechnology Journal 9, 373–383. [2]
- ^ Baur, A., R. Reski, G. Gorr (2005): Enhanced recovery of a secreted recombinant human growth factor using stabilizing additives and by co-expression of human serum albumin in the moss Physcomitrella patens. Plant Biotech. J. 3, 331–340 [3]
- ^ Protalix website – technology platform
- ^ Gali Weinreb and Koby Yeshayahou for Globes May 2, 2012. FDA approves Protalix Gaucher treatment
- ^ http://www.agf.gov.bc.ca/pesticides/infosheets/bt.pdf. Retrieved 21 January 2011
- ^ Hope, Alan (3 April 2013), News in brief: The Bio Safety Council...', Flanders Today, Page 2; In 2013, the Flemish Institute for Biotechnology was supervising a trial of 448poplar trees genetically engineered to produce less lignin so that they would be more suitable for conversion into bio-fuels.
- ^Nielsen, K. M. (2003). 'Transgenic organisms—time for conceptual diversification?” Nature Biotechnology 21 (3): 227–228. doi:10.1038/nbt0303-227. PMID 12610561.edit
- ^ Schouten, H.; Krens, F.; Jacobsen, E. (2006). 'Cisgenic plants are similar to traditionally bred plants: international regulations for genetically modified organisms should be altered to exempt cisgenesis'. EMBO Reports 7 (8): 750– 753.doi:10.1038/sj.embor.7400769. PMC 1525145. PMID 16880817. edit
- ^ Prins, T.W. and Kok, E.J. (2010) Food and feed safety aspects of cisgenic crop plant varieties Report 2010.001, Project number: 120.72.667.01, RIKILT – Institute of Food Safety, Netherlands. Retrieved 6 September 2010.
- ^ Melo, Eduardo O.; Canavessi, Aurea M. O.; Franco, Mauricio M.; Rumpf, Rodolpho (2007). 'Animal transgenesis: state of the art and applications'. J. Appl. Genet. 48(1): 47– 61. doi:10.1007/BF03194657. PMID 17272861. Archived from the original on 26 September 2009.
- ^ Leader, Benjamin; Baca, Qentin J.; Golan, David E. (January 2008). 'Protein therapeutics: a summary and pharmacological classification'. Nat Rev Drug Discov. A guide to drug discovery 7 (1): 21–39. doi:10.1038/nrd2399. PMID 18097458. Leader 2008 — Fee required for access to full text.
- ^ Walsh, Gary (April 2005). 'Therapeutic insulins and their large-scale manufacture'.Appl. Microbiol. Biotechnol. 67 (2): 151–159. doi:10.1007/s00253-004- 1809-x.PMID 15580495. Walsh 2005 — Fee required for access to full text
- ^ Summers, Rebecca (24 April 2013) Bacteria churn out first ever petrol-like biofuel New Scientist, Retrieved 27 April 2013
- ^ Pipe, Steven W. (May 2008). 'Recombinant clotting factors'. Thromb. Haemost. 99 (5): 840–850. doi:10.1160/TH07-10-0593. PMID 18449413.
- ^ Bryant, Jackie; Baxter, Louise; Cave, Carolyn B.; Milne, Ruairidh; Bryant, Jackie (2007). 'Recombinant growth hormone for idiopathic short stature in children and adolescents'. In Bryant, Jackie. Cochrane Database Syst Rev (3): CD004440. doi:10.1002/14651858.CD004440.pub2. PMID 17636758. Bryant 2007 — Fee required for access to full text
- ^ Baxter L, Bryant J, Cave CB, Milne R (2007). 'Recombinant growth hormone for children and adolescents with Turner syndrome'. In Bryant, Jackie. Cochrane Database Syst Rev (1): CD003887. doi:10.1002/14651858.CD003887.pub2.PMID 17253498.
- ^ Panesar, Pamit et al (2010) 'Enzymes in Food Processing: Fundamentals and Potential Applications', Chapter 10, I K International Publishing House, ISBN 978- 9380026336
- ^EFSA (2012). Genetically modified animals. Europe: EFSA. http://www.efsa.europa.eu/en/topics/topic/gmanimals.htm.
- ^ Murray, Joo (20). Genetically modified animals. Canada: Brainwaving. http://www.brainwaving.com/2010/07/28/genetically-modified-animals/.
- ^ Jaenisch, R. and Mintz, B. (1974). 'Simian virus 40 DNA sequences in DNA of healthy adult mice derived from preimplantation blastocysts injected with viral DNA.'. Proc. Natl. Acad. Sci. 71 (4): 1250–1254. Bibcode: 1974PNAS 71.1250J.doi:10.1073/pnas.71.4.1250. PMC 388203. PMID 4364530.
- ^ rudinko, larisa (20). Guidance for industry. USA: Center for veterinarymedicine Link.
- ^ Sathasivam K, Hobbs C, Mangiarini L, et al. (June 1999). [http: //journals.royalsociety.org /openurl.asp?genre=article&issn=0962- 8436& volume=354&issue=1386&spage=963 'Transgenic models of Huntington's disease']. Philos. Trans. R. Soc. Lond., B, Biol. Sci. 354 (1386):963–9. doi:10.1098/rstb.1999.0447. PMC 1692600. PMID 10434294.
- ^ Spencer, L; Humphries, J; Brantly, M. (12 May 2005). 'Antibody Response to Aerosolized Transgenic Human Alpha1-Antitrypsin'. New England Journal of Medicine 352: 19. Retrieved 28 April 2011.
- ^ 'The Nobel Prize in Chemistry 2008'. The Official Web Site of the Nobel Foundation. Retrieved 2012-08-31.
- ^ Cabot, R. A.; Kühholzer, B.; Chan, A. W. S.; Lai, L.; Park, K. -W.; Chong, K. - Y.; Schatten, G.; Murphy, C. N. et al. (2001). 'Transgenic Pigs Produced Using in Vitro Matured Oocytes Infected with a Retroviral Vector'. Animal Biotechnology 12 (2): 205– 214. doi:10.1081/ABIO-100108347. PMID 11808636. edit
- ^ Lai, L.; Park, K. W.; Cheong, H. T.; k Hholzer, B.; Samuel, M.; Bonk, A.; Im, G. S.; Rieke, A. et al. (2002). 'Transgenic pig expressing the enhanced green fluorescent protein produced by nuclear transfer using colchicine-treated fibroblasts as donor cells'. Molecular Reproduction and Development 62 (3): 300–306. doi:10.1002/mrd.10146. PMID 12112592. edit
- ^ Hogg, Chris (12 January 2006) Taiwan Breeds Green-Glowing Pigs BBC, Retrieved 31 August 2012
- ^ a b Staff (8 January 2008) Fluorescent Chinese pig passes on trait to offspring AFP, Retrieved 31 August 2012
- ^ a b Kawarasaki, T.; Uchiyama, K.; Hirao, A.; Azuma, S.; Otake, M.; Shibata, M.; Tsuchiya, S.; Enosawa, S. et al. (2009). 'Profile of new green fluorescent protein transgenic Jinhua pigs as an imaging source'. Journal of Biomedical Optics 14 (5): 054017. doi:10.1117/1.3241985. PMID 19895119. edit
- ^ a b Randall S. et al (2008) Genetically Modified Pigs for Medicine and Agriculture Biotechnology and Genetic Engineering Reviews – Vol. 25, 245–266, Retrieved 31 August 2012
- ^ Staff (2006) NTU produces green fluorescent pigs for medical research Taiwan Central News Agency, Retrieved 31 August 2012
- ^ Wongsrikeao P, Saenz D, Rinkoski T, Otoi T, Poeschla E (2011). 'Antiviral restriction factor transgenesis in the domestic cat'. Nature Methods 8 (10): 853–9. doi:10.1038/nmeth 1703. PMID 21909101.
- ^ Staff (3 April 2012) Biology of HIV National Institute of Allergy and InfectiousDiseases, Retrieved 31 August 2012.
- ^ Sasaki, E.; Suemizu, H.; Shimada, A.; Hanazawa, K.; Oiwa, R.; Kamioka, M.; Tomioka, I.; Sotomaru, Y. et al. (2009). 'Generation of transgenic non-human primates with germline transmission'. Nature 459 (7246): 523– 527. Bibcode: 2009 Natur.459..523S. doi:10.1038/nature08090. PMID 19478777. edit
- ^ Schatten, G.; Mitalipov, S. (2009). 'Developmental biology: Transgenic primate offspring'. Nature 459 (7246): 515–516. Bibcode:2009Natur.459..515S.doi:10.1038/459515a. PMC 2777739. PMID 19478771. edit
- ^ Cyranoski, D. (2009). 'Marmoset model takes centre stage'. Nature 459 (7246):492–492. doi:10.1038/459492a. PMID 19478751. edit
- ^ Louis-Marie Houdebine (2009) Production of Pharmaceutical by transgenic animals. Comparative Immunology, Microbiology & Infectious Diseases 32(2): 107– 121 [4]
- ^ Britt Erickson, 10 February 2009, for Chemical & Engineering News. FDA Approves Drug From Transgenic Goat Milk Accessed October 6, 2012
- ^ a b c d Guelph (2010). Enviropig. Canada: http://www.uoguelph.ca/enviropig/index.shtml/.
- ^ Schimdt, Sarah. Genetically engineered pigs killed after funding ends, Postmedia News, June 22, 2012. Accessed July 31, 2012.
- ^ a b Canada. 'Enviropig — Environmental Benefits | University of Guelph'. Uoguelph.ca. Retrieved 8 March 2010.
- ^ stevenson, heidi(2011). Scientists Use Human Genes in Animals, So CowsProduce Human-Like Milk—Or Do They? USA: http://www.gaia- health.com/articles401/000433- human-genes-cows-produce-human-milk.shtml/.
- ^ Classical Medicine Journal (14 April 2010). 'Genetically modified cows producing human milk.”
- ^ Yapp, Robin (11 June 2011). 'Scientists create cow that produces 'human' milk'. The Daily Telegraph (London). Retrieved 15 June 2012.
- ^ Jabed, A.; Wagner, S.; McCracken, J.; Wells, D. N.; Laible, G. (2012). 'Targeted microRNA expression in dairy cattle directs production of -lactoglobulin-free, high-casein milk'. Proceedings of the National Academy ofSciences. doi:10.1073/pnas.1210057109. edit
- ^ Lai L et al. (2006). 'Generation of cloned transgenic pigs rich in omega-3 fatty acids'. Nature Biotechnology 24 (4): 435–436. doi:10.1038/nbt1198.PMC 2976610. PMID 16565727. Retrieved 2009-03-29.
- ^ Zyga, Lisa (2010). Scientist bred goats that produce spider silk. Link.
- ^ a b {Aqu Advantage salmon}[5]
Back