Details

Substitution Trends in the Mitochondrial Cytochrome B Protein Coding Gene and the Corresponding Changes in its Amino Acid Sequence in Catfish Species

Mohd Imran

Dept. of Zoology, AMU, Aligarh, India

Arif Ahmad

Dept. of Zoology, MANUU, Gachibowli, India

Iqbal Parwez

Dept. of Zoology, AMU, Aligarh, India

11-18

Vol: 12, Issue: 3, 2022

Receiving Date: 2022-05-29 Acceptance Date:

2022-07-04

Publication Date:

This paper was withdrawn due to certain technical errors.


Abstract

The study describes the divergence trends in the partial segment cytochrome b gene (cyt. b) in six catfish species on the basis of the nucleotide substitutions and their location in the triplet codons of the resulting amino acid sequence. The nucleotide sequences of all six species were found A+T rich with average nucleotide frequencies as A: 28.6%; T: 29.3%; G: 13.6%; C: 28.5%, showing an anti-guanine bias which was found strongest in R. rita. The transition substitution rate was found highest at 3rd codon position in all the species. The evolutionary divergence follows the trend as highest at 3rd codon position followed by 1st codon position and the least on 2nd codon position among all species. When amino acid sequences of cytochrome b were aligned, only 12 sites were found with a total 29 amino acid substitutions. which is a resultant of the substitution trend of three codon positions. Majority of the amino acid substitutions were found synonymous and conservative. Three different variability trends are observed in the cyt. b gene which run simultaneously on three different codon positions of triplet codons. Nucleotide substitutions at three codon positions follows the order 3rd (87) >1st (32) >2nd (03) the frequency of amino acid substitution follows the order 1st (11) >3rd (04) >2nd (02); and the frequency of non-conservative amino acid changes found as 2nd (1:2) >1st (5:11) >3rd (1:4). The study has provided an in-depth understanding of the relative variability and substitution trends in the mitochondrial cytochrome b gene in catfish species at both DNA and protein level.

Keywords: cytochrome b gene; amino acid; triplet codon; divergence trend; substitutions

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